A nuclear gene of Saccharomyces cerevisiae needed for stable maintenance of plasmids.

EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE

The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...

Isolation, Subtype Determination, Cloning and Expression of HBsAg Gene from an Iranian Carrier in Saccharomyces cerevisiae

The Hepatitis B Surface antigen ( HBsAg) gene was isolated from an Iranian HBeAg positive carrier by PCR. The gene was cloned in pUC19 for sequencing and pYES2 for expression in Saccharomyces cerevisiae, which pNF1 and pDF3 constructs were made respectively. The sequencing data showed that the isolated HBsAg gene shared more than 90% homology with the ayw subtype. The pDF3 was transferred into ...

Inhibitory Effect of Supernatant and Lysate of Saccharomyces cerevisiae on Expression of exoA Gene of Pseudomonas aeruginosa

Background and Aim: Pseudomonas aeruginosa is an important ubiquitous and especially common pathogen in the hospital. Exotoxin A that encoded by exoA gene has a role in pathogenesis of this bacterium. Today, probiotics are widely used in the treatment and prevention of diseases. The present study aimed to study the Saccharomyces cerevisiae S3 effect on the expression of exoA gene. Materials an...

Disruption of GAL1 Gene in Saccharomyces cerevisiae Leads to Higher Ethanol Production

Effect of Processed Lemon Pulp With Saccharomyces Cerevisiae Yeast on Protein and Energy Metabolism in Raini Goats

The aim of present study is investigating effect of treated lemon pulp by Saccharomyces cerevisiae yeast on protein and energy metabolism in goats was fed with this product. In this experiment 8 goats from raini breed were used for 21 days period; 16 days for adaptation and 5 days for sampling, to investigate the effect of processing lemon pulp by Saccharomyces cerevisiae yeas...